A Practical Guide to Developmental Biology

About this book

This lab manual is designed to give students experience with a wide variety of model systems currently in use by developmental biologists. Experiments range from classic slide or whole animal observations to more modern immunohistochemistry and manipulation of gene expression.

Contents

TABLE OF CONTENTS; THE EXPERIMENTS; Introduction to Developmental Biology: Embryo Protocols, Ethics and Model Systems; Axial Patterning: Using Retinoic Acid to Disrupt Homeobox Gene Expression in Axolotls; Plant Cell Totipotency: Growing a Carrot from Adult Cells; Fertilization: Sea Urchin Syngamy & Development; Early Plant Development: Pollen Tube Formation; Morphogenesis: Creating Fate Maps of Albino Axolotls Using a Vital Dye; Cell Adhesion: Cell-Cell Interactions in Sponges & Amphibians; Embryogenesis: Chick & Amphibian Development (Slide-based); Neurulation: Labelling Chick Notochord using Immunocytochemistry; Gametogenesis: Comparison of Plants and Animals; Regeneration: Morphallaxis & Epimorphosis; Metamorphosis: Drosphila Imaginal Discs; External Influences on Development: Design Your Own Experiment with Amphibians, Chicks or Fish; INFORMATION FOR THE INSTRUCTOR; Animal Care & Maintenance; Animal & Plant Sources; Chemical Sources; Slides Needed / Sources; Solutions & Other Materials; GLOSSARY; LITERATURE CITED

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