Arabidopsis Protocols

About this book

A comprehensive collection of new and essential protocols containing many easily reproducible methods developed for use with arabidopsis. The methods range from the basics of growing these plants to sophisticated gene cloning strategies and can, in many cases, also be applied to other plant species with minor modifications.

Contents

Part I.Arabidopsis Culture. Growth of Plants and Preservation of Seeds Randy Scholl Luz Rivero-Lepinckas and Deborah Crist. Sterile Techniques in Arabidopsis Peter McCourt and Kallie Keith. Control of Pests and Diseases of Arabidopsis Mary Anderson. Establishment and Maintenance of Cell Suspension Cultures Jaideep Mathur and Csaba Koncz. Callus Culture and Regeneration Jaideep Mathur and Csaba Koncz. Protoplast Isolation Culture and Regeneration Jaideep Mathur and Csaba Koncz. Part II.Purification of Subcellular Organelles and Macromolecules. Preparation of Physiologically Active Chloroplasts from Arabidopsis Ljerka Kunst. Purification of Mitochondria from Arabidopsis Mathieu Klein Stefan Binder and Axel Brennicke. Preparation of DNA from Arabidopsis Jianming Li and Joanne Chory. High Molecular Weight DNA Extraction from Arabidopsis David Bouchez and Christine Camilleri . Chloroplast DNA Isolation George S. Mourad . Purification of Mitochondrial DNA from Green Tissues of Arabidopsis Mathieu Klein Rudolf Hiesel Charles Andr' and Axel Brennicke. Preparation of RNA Clifford D. Carpenter and Anne E. Simon. Part III.Mutagenesis and Genetic Analysis. Seed Mutagenesis of Arabidopsis Jonathan Lightner and Timothy Caspar. Genetic Analysis Maarten Koornneef Carlos Alonso-Blanco and Piet Stam. Cytogenetic Analysis of Arabidopsis John S. Heslop-Harrison. PCR-Based Identification of T-DNA Insertion Mutants Rodney G. Winkler and Kenneth A. Feldmann . Part IV.Gene Mapping in Arabidopsis. The Use of Recombinant Inbred Lines (RILs) for Genetic Mapping Carlos Alonso- Blanco Maarten Koornneef and Piet Stam. AFLPTM Fingerprinting of Arabidopsis Pieter Vos. Building a High-Density Genetic Map Using the AFLPTM Technology Martin T. R. Kuiper. Use of Cleaved Amplified Polymorphic Sequences (CAPS) as Genetic Markers in Arabidopsis thaliana Jane Glazebrook Eliana Drenkard Daphne Preuss and Frederick Ausubel . Mapping Mutations with ARMS Anton R. Sch"ffner. Mapping Cloned Sequences on YACs Francis D. Agyare Gus Lagos Deval Lashkari Ronald W. Davis and Bertrand Lemieux. Part V.Transient and Stable Transformation. Transient Gene Expression in Protoplasts of Arabidopsis thaliana Steffen Abel and Athanasios Theologis. Transient Expression of Foreign Genes in Tissues of Arabidopsis thaliana by Bombardment-Mediated Transformation Motoaki Seki Asako Iida and Hiromichi Morikawa. Root Transformation by Agrobacterium tumefaciens Annette C. Vergunst Ellen C. de Waal and Paul J. J. Hooykaas . Transformation of Arabidopsis thaliana C Leaf Discs by Agrobacterium tumefaciens Eric van der Graaf and Paul J. J. Hooykas . In Planta Agrobacterium-Mediated Transformation of Adult Arabidopsis thaliana Plants by Vacuum Infiltration Nicole Bechtold and Georges Pelletier . PEG- Mediated Protoplast Transformation with Naked DNA Jaideep Mathur and Csaba Koncz. Part VI. Gene Cloning Strategies. Cloning Genes of Arabidopsis thaliana by Chromosome Walking Jeffrey Leung and J'rme Giraudat. Chromosome Landing Using an AFLPTM-Based Strategy Ann Van Gysel Gerda Cnops Peter Breyne Marc Van Montagu and Maria Teresa Cervera. Transposon Tagging with Ac/Ds in Arabidopsis Deborah Long and George Coupland . Transposon Tagging with the EN-I System Andy Pereira and Mark G. M. Aarts. Cloning Genes from T-DNA Tagged Mutants Brian P. Dilkes and Kenneth A. Feldmann . Part VII.Gene Expression Analyses. In Situ Hybridization Gary N. Drews. Whole-Mount In Situ Hybridization in Plants Janice de Almeida Engler Marc Van Montagu and Gilbert Engler . Bacterial and Coelenterate Luciferases as Reporter Genes in Plant Cells William H. R. Langridge and Aladar A. Szalay . b-Glucoronidase Enzyme Histochemistry on Semithin Sections of Plastic-Embedded Arabidopsis Explants Marc De Block and Mieke Van Lijsebettens . In Situ Hybridization to RNA in Whole Arabidopsis Plants Qingzhong Kong and Anne E. Simon. In Vivo Footprinting in Arabidopsis Anna-Lisa Paul and Robert J. Ferl. Index.

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