DNA Science A First Course

About this book

The second edition of this text contains a collection of 12 tested experiments in molecular biology, developed at the Dolan DNA Learning Center of Cold Spring Harbor Laboratory, which culminate in the construction and cloning of a recombinant DNA molecule. Coverage also includes the completion of the draft sequence of the human genome and enormous impact these and other sequence data are having on medicine, agriculture and industry. The laboratory experiments cover basic techniques of gene isolation and analysis, explained sufficiently to be useful to teachers and students with no prior experience in the field. Prelab notes at the beginning of each experiment explain how to schedule and prepare, while flow charts and icons should make the protocols easy to follow.

Contents

How we learned that DNA is the genetic material; how we learned how genes are regulated; basic tools and techniques of DNA science; methods for finding and expressing important genes; modern methods for analyzing whole genomes; the DNA science of cancer; applying DNA science to human genetics and evolution. Labs - lab safety and adherence to national institutes of health guidelines; measurements, micropipetting, and sterile techniques; bacterial culture techniques; DNA restriction analysis; effects of DNA methylation on restriction; rapid colony transformation of E. coli with plasmid DNA; assay for an antibiotic resistance enzyme; purification and identification of recombinant GFP; purification and identification of plasmid DNA; recombination of antibiotic resistance genes; transformation of E. coli with recombinant DNA; replica plating to identify mixed E. coli populations; purification and identification of recombinant DNA; answers to discussion questions. Appendices - equipment, supplies and reagents; recipes for media, reagents and stock solutions; restriction map data for pAMP, pKAN, pBLU, GFP and bacteriophage; cautions.

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