Fungal Diagnostics Methods and Protocols

About this book

Invasive fungal infections are a significant cause of morbidity and mortality. Over the past decade there has been a concerted effort to develop reliable methods for the detection of such infections. In Fungal Diagnostics: Methods and Protocols, expert researchers in the field detail the introduction of new technology into a diagnostic setting include ease of use, and rapid turnaround time without compromising sensitivity and specificity.

Focusing specifically on fungal detection in clinical settings, fungal diagnostics including, environmental testing, agriculture and food production and veterinary diagnostics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Fungal Diagnostics: Methods and Protocols seeks to aid scientists into the further study of diagnostic and detection of fungi.

Contents

1. Overview of Invasive Fungal Infections Nina L. Tuite and Katrina Lacey
2. Fungal Diagnostics: Review of Commercially Available Methods Javier Yugueros Marcos and David H. Pincus
3. Rapid Methods for the Extraction and Archiving of Molecular Grade Fungal Genomic DNA Andrew M. Borman, Michael Palmer and Elizabeth M. Johnson
4. Apex DNA Microarray for the Identification of Pathogenic Fungi Arianna Tavanti, Stefano Landi, and Sonia Senesi
5. Microscopic Detection of Yeasts using Fluorescence in situ Hybridization Joao Inacio and Maria da Luz Martins
6. Quantitative Detection of Aspergillus spp. by Real-time Nucleic Acid Sequence-Based Amplification (NASBA) Yanan Zhao and David S. Perlin
7. Differentiation of Fungi using Hybridization Probes on the LightCycler(R) Stephan Fricke, Nadja Hilger, Christopher Oelkrug, Christian Fricke
8. Quantitative and Multiplex Detection of Pathogenic Fungi using Padlock Probes, Generic qPCR and Suspension Array Readout Magnus Jobs, Ronnie Eriksson and Jonas Blomberg
9. Species-Specific Identification of a Wide Range of Clinically Relevant Fungal Pathogens by the Luminex xMAP Technology Preuner S., Lion T
10. Applied Gene Histopathology: Identification of Fusarium Species in FFPE Tissue Sections by in situ Hybridization Yoichiro Okubo, Minoru Shinozaki, Megumi Wakayama, Haruo Nakayama, Daisuke Sasai, Takao Ishiwatari, Tetsuo Nemoto, and Kazutoshi Shibuya
11. Application of Chip-based Flow Cytometry for Amphotericin B and Fluconazole Susceptibility Testing on Candida Strains Orsolya Bouquet, Bela Kocsis, Ferenc Kilar, and Ildiko Kustos
12. Surface Plasmon Resonance Genosensor for the Detection of Fusarium culmorum Michelangelo Pascale, Francesco Zezza, and Giancarlo Perrone
13. Hyperbranching Rolling Circle Amplification (HRCA), An Improved Protocol for Discriminating Between Closely Related Fungal Species Jiufeng Sun and Sybren de Hoog
14. Loop-mediated Isothermal Amplification Based Detection of Fusarium graminearum Ludwig Niessen
15. Multiplex-Tandem PCR for Fungal Diagnostics Anna Lau, Keith Stanley, and Tania Sorrell
16. Specific Detection of Pneumocystis jirovecii in Clinical Samples by Flow Cytometry Joana Barbosa, Sofia Costa-de-Oliveira, Ana Teresa Silva, Acacio Goncalves Rodrigues, Cidalia Pina-Vaz

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