Laboratory Experiments in Microbiology (International Edition)

About this book

Containing 57 thoroughly class-tested and easily customizable exercises, Laboratory Experiements in Microbiology (Tenth Edition) provides engaging labs with instruction on performing basic microbiology techniques and applications for undergraduate students in diverse areas, including the biological sciences, the allied health sciences, agriculture, environmental science, nutrition, pharmacy, and various pre-professional programs. The Tenth Edition features an updated art program and a full-color design, integrating valuable micrographs throughout each exercise. Additionally, many of the illustrations have been re-rendered in a modern, realistic, three-dimensional style to better visually engage students. Laboratory Reports for each exercise have been enhanced with new Clinical Applications questions, as well as question relating to Hypotheses or Expected Results. Experiments have been refined throughout the manual and the Tenth Edition includes an extensively revised exercise on transformation in bacteria using pGLO to introduce students to this important technique.

Contents

Introduction

I. MICROSCOPY
1. Use and Care of the Microscope
2. Examination of Living Microorganisms

II. Handling Bacteria
3. Microbes in the Environment
4. Transfer of Bacteria: Aseptic Techniques

III. STAINING METHODS
5. Preparation of Smears and Simple Staining
6. Negative Staining
7. Gram Staining
8. Acid-Fast Staining
9. Structural Stains (Endospore, Capsule, Flagella)
10. Morphologic Unknown

IV. CULTIVATION OF BACTERIA
11. Isolation of Bacteria by Dilution Techniques
12. Special Media for Isolating Bacteria

V. MICROBIAL METABOLISM
13. Carbohydrate Catabolism
14. Fermentation
15. Protein Catabolism, Part 1
16. Protein Catabolism, Part 2
17. Respiration
18. Unknown Identification and Bergey’s Manual

VI. MICROBIAL GROWTH
19. Oxygen and the Growth of Bacteria
20. Determination of a Bacterial Growth Curve: The Role of Temperature
21. Biofilms

VII. CONTROL OF MICROBIAL GROWTH
22. Physical Methods of Control: Heat
23. Physical Methods of Control: Ultraviolet Radiation
24. Chemical Methods of Control: Disinfectants and Antiseptics
25. Chemical Methods of Control: Antimicrobial Drugs
26. Effectiveness of Hand Scrubbing

VIII. MICROBIAL GENETICS
27. Regulation of Gene Expression
28. Isolation of Bacterial Mutants
29. Transformation of Bacteria
30. DNA Fingerprinting
31. Genetic Engineering
32. Ames Test for Detecting Possible Chemical Carcinogens

IX. THE MICROBIAL WORLD
33. Fungi: Yeasts and Molds
34. Phototrophs: Algae and Cyanobacteria
35. Protozoa
36. Parasitic Helminths

X. VIRUSES
37. Isolation and Titration of Bacteriophages
38. Plant Viruses

XI. INTERACTION OF MICROBE AND HOST
39. Epidemiology
40. Koch’s Postulates

XII. IMMUNOLOGY
41. Innate Immunology
42. Agglutination Reactions: Slide Agglutination
43. Agglutination Reactions: Microtiter Agglutination
44. ELISA Technique

XIII. MICROORGANISMS AND DISEASE
45. Bacteria of the Skin
46. Bacteria of the Respiratory Tract
47. Bacteria of the Mouth
48. Bacteria of the Gastrointestinal Tract
49. Bacteria of the Genitourinary Tract
50. Identification of an Unknown from a Clinical Sample
51. Rapid Identification Methods

XIV. MICROBIOLOGY AND THE ENVIRONMENT
52. Microbes in Water: Multiple-Tube Technique
53. Microbes in Water: Membrane Filter Technique
54. Microbes in Food: Contamination
55. Microbes Used in the Production of Foods
56. Microbes in Soil: The Nitrogen and Sulfur Cycles
57. Microbes in Soil: Bioremediaton

APPENDICES
A. Pipetting
B. Dilution Techniques and Calculations
C. Use of the Spectrophotometer
D. Graphing
E. Use of the Dissecting Membrane
F. Use of the Membrane Filter
G. Electrophoresis
H. Keys to Bacteria

Index

Customer Reviews

Biography

Ted R. Johnson is a professor of biology at St. Olaf College, a liberal arts college in Northfield, Minnesota, where he teaches courses in microbiology and immunology. He has taught at St. Olaf for 34 years and previously taught at Mankato State University. He received his master’s degree and his Ph.D in microbiology from the University of Illinois in Chicago, Illinois. While at St. Olaf College, he has developed and directed several abroad semester and interim programs. His research focuses on the immune response to virally induced cancer in various animal models.

Christine L. Case is a registered microbiologist and a professor of microbiology at Skyline College in San Bruno, California, where she has taught for the past 41 years. She received her Ed. D. in curriculum and instruction from Nova Southeastern University and her M.A. in microbiology from San Francisco State University. She was Director for the Society for Industrial Microbiology (SIM). She received the ASM and California Hayward outstanding educator awards and SACNAS Mentor award. In addition to teaching, Chris contributes regularly to professional literature, develops innovative educational methodologies, and maintains a personal and professional commitment to conservation and the importance of science in society. Chris is also an avid photographer, and many of her photographs appear in this lab manual.