Plant Cell Culture: A Practical Approach

About this book

Plant cell culture techniques are being increasingly used in basic research and for plant exploitation in industry (genetic engineering, micropropagation). In this book, internationally recognized experts describe the techniques in use in their laboratories for isolating and manipulating cell cultures, and their central importance in plant biotechnology. This book is intended for postgraduate researchers, college lecturers, industrial/biotechnology researchers in plant sciences.

Contents

1. Isolation and maintenance of callus and cell suspension cultures; 2. Isolation, culture, and regeneration of protoplasts; 3. Applications of protoplast technology; 3.A Fusion and selection of somatic hybrids; 3.B Transient gene expression and stable transformation; 3.C Studies with viruses; 4. Selection of plant cells for desirable characteristics; 4.A Inhibitor resistance; 4.B Cold tolerance; 4.C In vitro selection for salt tolerance; 4.D In vitro selection for disease/toxin resistance; 5. Plant regeneration via embryonic suspension cultures; 6. Applied aspects of plant regeneration; 6.A Micropropagation; 6.B Virus-free plants; 6.C Artificial seeds; 7. Cryopreservation; 8. Secondary products from cultured cells and organs I: molecular and cellular approaches; 9. Secondary products from cultured cells and organs II: large scale culture

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