Plant Hormone Protocols

About this book

Established investigators from around the world describe in step-by-step detail their best techniques for the study of plant hormones and their regulatory activities. These state-of-the-art methods include contemporary approaches to identifying the biosynthetic pathways of plant hormones, monitoring their levels, characterizing the receptors with which they interact, and analyzing the signaling systems by which they exert their effects. Comprehensive and fully detailed for reproducible laboratory success, Plant Hormone Protocols offers plant biologists an indispensable compendium of today's most powerful methods and strategies to studying plant hormones, their regulation, and their activities.

Contents

Extraction and Purification of an Enzyme Potentially Involved in ABA Biosynthesis. Differential Display: Analysis of Gene Expression During Plant Development. Abscisic Acid: ABA Immunoassay and Gas Chromatography/Mass Spectrometry Verification. Auxin Analysis. Photoacoustic and Photothermal Detection of the Plant Hormone Ethylene. Analysis of Gibberellins. Cytokinins: Extraction, Separation, and Analysis. Binding Studies. Mutagenesis. The Identification of Ethene Biosynthetic Genes by Gene Silencing: Antisense Transgenes, Agrobacterium-Mediated Transformation, and the Tomato ACC Oxidase cDNA. Extraction, Separation, and Analysis of Plant Phosphoinositides and Complex Glycolipids. Reverse Genetics: Screening Plant Populations for Gene Knockouts. Index.

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