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About this book
Revised and expanded, this edition remains the first source to turn for a complete summary of tried and tested protein techniques.
Contents
Preparation for Protein Isolation. Protein Extraction and Solubilization. Protein Concentration Determination. Concentrating Protein Solutions. Gel Electrophoresis Under Denaturing Conditions. Gel Electrophoresis Under Nondenaturing Conditions. Isoelectric Focusing and Two--Dimensional Gel Electrophoresis. Immunoblotting. Ion Exchange Chromatography. Gel Filtration Chromatography. Affinity Chromatography. Hanging Drop Crystallization. Appendices. Index
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