The Plant Cell Wall Methods and Protocols

About this book

The cell wall and its constituent polysaccharides and proteins control nearly all plant-based biological and biophysical processes. Understanding the cell wall is, therefore, not only fundamental to the plant sciences but is also pertinent to aspects of human and animal nutrition and health as well as plant-microbe and plant-animal interactions.

In "The Plant Cell Wall: Methods and Protocols", experts in the field describe detailed methods which are currently being applied to investigate the many aspects of the plant cell wall including its structure, biochemical composition, and metabolism. The book delves into a range of techniques involving plant tissue culture, which can be applied to investigating cell wall structure and metabolism, methods directed towards structural analysis and occurrence of carbohydrates, the development and use of microscopy-based tools and techniques, procedures which measure the physical properties of the wall, and methods based on the application of molecular genetic approaches.

Contents

1. Plant Tissue Cultures Anna Karkonen, Arja Santanen, Kuninori Iwamoto, and Hiroo Fukuda 2. Computerized Molecular Modelling of Carbohydrates Alfred D. French and Glenn P. Johnson 3. Oligosaccharide Mass Profiling (OLIMP) of Cell Wall Polysaccharides by MALDI-TOF/MS Markus Gunl, Florian Kraemer, and Markus Pauly 4. High-Voltage Paper Electrophoresis (HVPE) of Cell-Wall Building Blocks and Their Metabolic Precursors Stephen C. Fry 5. Carbohydrate Gel Electrophoresis Florence Goubet, Paul Dupree, and Katja Salomon Johansen 6. Capillary Electrophoresis with Detection by Laser-Induced Fluorescence Andrew Mort and Xiangmei Wu 7. Monoclonal antibodies, Carbohydrate-Binding Modules, and the Detection of Polysaccharides in Plant Cell Walls Cecile Herve, Susan E. Marcus, and J. Paul Knox 8. Screening and Characterization of Plant Cell Walls Using Carbohydrate Microarrays Iben Sorensen and William G.T. Willats 9. Electron Tomography and Immunogold Labelling as Tools to Analyze de novo Assembly of Plant Cell Walls Marisa S. Otegui 10. Analyzing Cellulose Biosynthesis with Confocal Microscopy Meera Nair and Seth DeBolt 11. Visual Mapping of Cell Wall Biosynthesis Yumiko Sakuragi, Morten H.H. Norholm, and Henrik V. Scheller 12. Atomic Force Microscopy of Plant Cell Walls Andrew R. Kirby 13. Using Solid-State 13C NMR Spectroscopy to Study the Molecular Organization of Primary Plant Cell Walls Tracey J. Bootten, Philip J. Harris, Laurence D. Melton, and Roger H. Newman 14. Formation of Cellulose-Based Composites with Hemicelluloses and Pectins Using Gluconacetobacter Fermentation Deirdre Mikkelsen and Michael J. Gidley 15. Structural Proteins of the Primary Cell Wall: Extraction, Purification, and Analysis Derek T.A. Lamport, Li Tan, and Marcia J. Kieliszewski 16. New Insights into the Control of Cell Growth Claudia Blaukopf, Matthaus Z. Krol, and Georg J. Seifert 17. Extraction and Detection of Arabinogalactan Proteins Zoe A. Popper 18. Characterization of the Plant Cell Wall Proteome Using High Throughput Screens Sang-Jik Lee and Jocelyn K.C. Rose 19. Knocking Out the Wall: Protocols for Gene Targeting in Physcomitrella patens Alison W. Roberts, Christos S. Dimos, Michael J. Budziszek, Jr., Chessa A. Goss, and Virginia Lai 20. Measuring In vitro Extensibility of Growing Plant Cell Walls Daniel J. Cosgrove

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