Transgenic Techniques

About this book

This revised and updated edition of a much-acclaimed classic presents a collection of readily reproducible techniques for the generation and analysis of transgenic animals, with emphasis on the manipulation of the mammalian genome. It concentrates on the creation and maintenance of genetically modified murine strains, providing access to the germline by both conventional pronuclear injection, and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms.

Contents

Biology and Regulation of the Matrix Metalloproteinases, Substrate Specificity of Matrix Metalloproteinases, The Tissue Inhibitors of Metalloproteinases (TIMPs): Biology and Regulation, Matrix Metalloproteinases in Cancer, Hydroxamic Acid Matrix Metalloproteinase Inhibitors, Pinomastat, A Potent and Selective Matrix Metalloproteinase Inhibitor: Preclinical and Clinical Development for Oncology, A Potent Non-Peptidic Matrix Metalloproteinase Inhibitor: Discovery of BAY 12-9566, D1927 and D2163: Novel Mercaptoamide Inhibitors of Matrix Metalloproteinases, Matrix Metalloproteinase Inhibitor Research at Boehringer Mannheim, Therapeutic Applications of Matrix Metalloproteinase Inhibitors Outside of Oncology,

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