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Basic Life Science Methods: A Laboratory Manual for Students and Researchers presents forty of the most executed life science assays. The authors use a consistent structure to cover the preparation, execution and analysis of data from each method. Assays include estimation of cholesterol fractions, C-Reactive Protein, Genomic DNA isolation, Agarose Gel Electrophoresis, RT-PCR, DNA solution preparation, how to design primers, and enzyme-linked immunosorbent assay (ELISA). This book provides a complete reference containing step-by-step instructions on how to run life science assays. Laboratory staff can also benefit from the book as a training resource.
1. Calculations of Molarity and Normality
2. Glassware washing and sterilization techniques
3. pH Meter: Its use and calibration
4. Principle and working of Autoclave
5. Principle and working of Centrifuge
6. Principles and working of Laminar
7. Estimation of Total cholesterol (Flegg 1972)
8. Estimation of Creatinine
9. Estimation of blood Urea
10. Estimation of blood glucose By GOD – POD method
11. Determination of Albumin, and Globulin in Plasma
12. Quantitative determination of Homocysteine
13. Estimation of serum bilirubin
14. Estimation of diagnostic enzyme ALT
15. Estimation of diagnostic enzyme AST
16. To Estimate Protein by Lowry’s Method
17. Estimation of Reducing sugar by using Dinitro Salicylic Acid (DNS) Method
18. To perform quantitative determination of total protein in Serum/plasma
19. Enzyme Activity Using Starch assay
20. To Estimate Protein by Bradford’s Assay
21. To Identify a Protein Sample Using SDS-PAGE
22. Genomic DNA isolation from whole blood
23. PCR for gene amplification
24. Agarose Gel Electrophoresis (AGE)
25. RNA Isolation from Human tissue
26. Real-time polymerase chain reaction (RT-qPCR)
27. Preparation of competent cells by CaCl2 treatment
28. RNA Isolation from Plant tissues
29. DNA isolation from the bacterial culture broth
30. DNA isolation from fungal Mycelium
31. Quantification of the DNA using spectrophotometer
32. Formaldehyde gel electrophoresis of the isolated RNA sample
33. Paper chromatography of different amino acid
34. Separation of plants pigments using Column Chromatography
35. Separation of plant pigment by Thin Layer Chromatography
36. Chromatin Immunoprecipitation (ChIP) Protocol
37. Types of Media
38. Preparation of Nutrient Agar Media
39. Serial Dilution of the Bacterial Cells
40. Spread Plate Method of The Bacterial Cells
41. Streak Plate Method
42. Identified Bacterial Colony in Culture Broth
43. Pour plate method for bacterial colony counting
44. Gram Staining of Bacteria
45. Casein hydrolysis test
46. Antibiotic resistance test by Agar Well Diffusion Method
Dr Javeed Ahmad Tantray’s current research focus is on Human cardiovascular diseases obtained his PhD from Osmania University in Hyderabad, India. Dr Tantray has done Post Doc studies with the Department of Zoology, University of Kashmir, receiving funding from the UGC-CSIR. He published many National and International peer-reviewed research articles. Dr Tantray has published in high-impact factor journals such as the International Journal of Cardiology, and the Saudi Journal of Biological Science. He has guided 50, M.Sc and graduate students projects. He has received Junior scientist awards both at the national and international level. He received the Best Abstract Award at the 4th Asia Pacific CardioMetabolic Syndrome Congress held by the Korean Society of CardioMetabolic Syndrome, with an award amount of USD 500. Dr Tantray has organized a national seminar on "Cancer Bio-markers" and a one-day national workshop on “Assembly and usage of Foldscope”. Dr Tantray is currently an Assistant Professor in the Department of Zoology, School of life sciences central university of Kashmir (J&K).
Dr Sheikh Mansoor Shafi is a postdoctoral researcher, presently working at the SK- Institute of Medical Sciences Soura Srinagar, India. Previously he was working as a project associate-I at the CSIR-Indian Institute of Integrative Medicine Jammu (IIIM). He obtained his PhD from Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu. Dr Mansoor has made important contributions to his field and has an impressive lab experience in biochemistry, molecular biology, and genetics. He has published more than 30 research and review papers in reputed journals. He has published more than 10 book chapters, 3 books and he has filed one patent application.
Rasy Fayaz Choh Wani PhD has completed her M.Sc. degree from Kashmir University. Ms Rasy is currently pursuing PhD. She has published her research work in many reputed journals. She has presented her different papers in different international and national conferences. She is currently on the research topic “Isolation and characterization of pathogenic bacterial microflora associated with cultured Cyprinus carpio in Kashmir Valley”.
Nighat Un Nissa has completed her M.Sc. and M.Phil. From the Department of Zoology, University of Kashmir. Ms Nissa is currently pursuing her PhD in Zoology and is working on Fish parasites and their impact on pollution. She has published her research work in National and International journals. She was awarded Gold Medal for poster presentation at the Animal science congress held by the Department of Zoology, University of Kashmir.
