Plant bacterial diseases are a major factor in economic crop losses worldwide. The transportation and use of clean, pathogen-free seed and propagative material is the most vital step in controlling bacterial diseases, but this requires accurate, rapid, sensitive, and reliable pathogen detection.
The all-new second edition of Detection of Plant-Pathogenic Bacteria in Seed and Other Planting Material is an essential, easy-to-use lab manual describing and illustrating the latest methods for detecting a wide range of bacteria responsible for many of the world's most economically significant bacterial plant diseases in crops.
Yet this book does more than describe and illustrate the use of real-time PCR, rapid PCR-based assays, and other methods for specific, sensitive detection of bacteria in seed and propagative material. Its 48 chapters offer background information on the given bacteria and the diseases they cause, describe symptoms, detail their epidemiology, identify strategies for disease management, and provide protocols for detection in thorough detail.
The second edition of this comprehensive reference, published more than 25 years after the first edition, is organized into four parts:
- General Seed Pathology
- Detection of Plant-Pathogenic Bacteria in Cereals, Grain, Legumes, Grasses, and Forage
- Detection of Plant-Pathogenic Bacteria in Vegetables
- Detection of Plant-Pathogenic Bacteria in Vegetatively Propagated Crops
In addition to serving as a vital resource for seed companies, quarantine operations, and diagnostic labs, Detection of Plant-Pathogenic Bacteria in Seed and Other Planting Material is an excellent laboratory guide for classroom instruction. It can also help facilitate rapid technology transfer in developing countries, where opportunities to receive training in this important field are rare.
PART I: General Seed Pathology
CHAPTER 1. Bacterial Diseases of Plants: Epidemiology and Management Strategies
M’Barek Fatmi and Hasan Bolkan
CHAPTER 2. Taxonomy of Plant-Pathogenic Bacteria
Norman W. Schaad, Erin Schuenzel, and Jeffrey B. Jones
CHAPTER 3. Review of Seed Sample Size Determination and Methods for Extracting Phytopathogenic Bacteria from Seeds
Ron R. Walcott and Ron D. Gitaitis
PART II: Detection of Plant-Pathogenic Bacteria in Cereals, Grain, Legumes, Grasses, and Forage
CHAPTER 4. Detection of Pseudomonas syringae pv. syringae in Wheat Seeds
Siham Asaad, David C. Sands, and S. Krishna Mohan
CHAPTER 5. Detection of Xanthomonas translucens in Wheat Seeds
Etienne Duveiller and Claude Bragard
CHAPTER 6. Detection of Pantoea stewartii subsp. stewartii in Maize Seeds
Charles C. Block, Lisa M. Shepherd, and Jerald K. Pataky
CHAPTER 7. Detection of Clavibacter michiganensis subsp. nebraskensis in Maize Seeds
Lisa M. Shepherd, Charles C. Block, and Anne K. Vidaver
CHAPTER 8. Detection of Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola in Rice Seeds
Casiana M. Vera Cruz, Bart Cottyn, Marian Hanna Nguyen, Jillian Lang, Valérie Verdier, Twng Wah Mew, and Jan E. Leach
CHAPTER 9. Detection of Acidovorax oryzae in Rice Seeds
Tingchang Zhao, Wei Guan, Laixin Luo, Jianqiang Li, Jianjun Feng, and Norman W. Schaad
CHAPTER 10. Detection of Xanthomonas campestris pv. phaseoli and X. fuscans subsp. fuscans in Common Bean Seeds
Robert L. Gilbertson, Michael O’Leary, Irina V. Agarkova, and Anne K. Vidaver
CHAPTER 11. Detection of Pseudomonas syringae pv. phaseolicola in Bean Seeds
S. Krishna Mohan, Yvette Berthier-Schaad, and Norman W. Schaad
CHAPTER 12. Detection of Curtobacterium flaccumfaciens pv. flaccumfaciens in Bean Seeds and in Seeds of Other Leguminosae Crops
Stefania Tegli, Matteo Cerboneschi, and Anne K. Vidaver
CHAPTER 13. Detection of Pseudomonas savastanoi pv. glycinea in Soybean Seeds
Lisa M. Shepherd and Charles C. Block
CHAPTER 14. Detection of Rhodococcus fascians in Chickpea Seeds
Irina V. Agarkova and Anne K. Vidaver
CHAPTER 15. Detection of Rathayibacter spp. in Seeds of Cereals and Grasses
Elena Postnikova, Irina V. Agarkova, William L. Schneider, Aaron J. Sechler, and Ian T. Riley
CHAPTER 16. Detection of Clavibacter michiganensis subsp. insidiosus in Alfalfa Seeds
Deborah A. Samac, Kathy Ophel-Keller, and David Caffier
PART III: Detection of Plant-Pathogenic Bacteria in Vegetables
CHAPTER 17. Detection of Clavibacter michiganensis subsp. michiganensis in Tomato Seeds
M’Barek Fatmi, Hasan Bolkan, and Norman W. Schaad
CHAPTER 18. Detection of Pseudomonas syringae pv. tomato in Tomato Seeds
Parm Randhawa, Prakash Pradhanang, and Norman W. Schaad
CHAPTER 19. Detection of Xanthomonas spp. in Tomato and Pepper Seeds
Sally A. Miller, Jeffrey B. Jones, and Chester Kurowski
CHAPTER 20. Detection of Xanthomonas hortorum pv. carotae in Carrot Seeds
Lindsey J. du Toit, Robert L. Gilbertson, Margreet Asma, and Norman W. Schaad
CHAPTER 21. Detection of Pantoea spp. in Onion Seeds
Teresa A. Coutinho and Ron R. Walcott
CHAPTER 22. Detection of Burkholderia cepacia in Onion Planting Materials and Onion Seeds
Ali M. Zaid, Jo Ann E. Asselin, and Steven V. Beer
CHAPTER 23. Detection of Xanthomonas campestris pv. campestris in Crucifer Seeds
Harrie Koenraadt, Deborah L. Hailstones, Alexander Ignatov, and Norman W. Schaad
CHAPTER 24. Detection of Pseudomonas Pathogens from Crucifer Seeds
Carolee T. Bull and Steven T. Koike
CHAPTER 25. Detection of Xanthomonas campestris pv. vitians in Lettuce Seeds
Steven T. Koike and Robert L. Gilbertson
CHAPTER 26. Detection of Acidovorax citrulli in Cucurbit Seeds
Ron R. Walcott, Jianjun Feng, and Ron D. Gitaitis
CHAPTER 27. Detection of Pseudomonas syringae pv. lachrymans in Cucumber Seeds
Carmen N. Mortensen and M’Barek Fatmi
CHAPTER 28. Detection of Pantoea ananatis in Melon Seeds
Kazutaka Kido and Yuichi Takikawa
PART IV: Detection of Plant-Pathogenic Bacteria in Vegetatively Propagated Crops
CHAPTER 29. Detection of Clavibacter michiganensis subsp. sepedonicus in Potato Tubers
Solke H. De Boer, John G. Elphinstone, and Norman W. Schaad
CHAPTER 30. Detection of Ralstonia solanacearum in Potato and Geranium
X. Li, Solke H. De Boer, Anne M. Alvarez, and Norman W. Schaad
CHAPTER 31. Detection of Common Scab–Causing Streptomyces in Potato Tubers and Soil
Leslie A. Wanner and M’Barek Fatmi
CHAPTER 32. Detection of ‘Candidatus Liberibacter solanacearum’ in Potato
Wenbin Li, Jorge A. Abad, Aimin Wen, Neil C. Gudmestad, Jacob A. Price, and Charlie M. Rush
CHAPTER 33. Detection of Pectobacterium spp. and Dickeya spp. in Potato Tubers
Solke H. De Boer, Amy O. Charkowski, and Jan M. van der Wolf
CHAPTER 34. Detection of Xanthomonas fragariae in Strawberry Planting Stock
Parm Randhawa and Edwin Civerolo
CHAPTER 35. Detection of ‘Candidatus Liberibacter asiaticus’ in Citrus Planting Materials
Yongping Duan and Wenbin Li
CHAPTER 36. Detection of Xanthomonas citri subsp. citri in Citrus
U. Lertsuchatavanich, J. Cubero, N. Thaveechai, and P. Llop
CHAPTER 37. Detection of Xylella fastidiosa in Grape, Almond, and Citrus
M’Barek Fatmi, Jianchi C. Chen, and Norman W. Schaad
CHAPTER 38. Detection of Xylella fastidiosa subsp. multiplex in Peach, Plum, and Shade Trees
C. J. Chang, Frank Wong, and Paul Vincelli
CHAPTER 39. Detection of Xylella fastidiosa subsp. sandyi in Oleander
Donald A. Cooksey and Hamid R. Azad
CHAPTER 40. Detection of Erwinia amylovora in Asymptomatic Budwood of Pear, Apple, and Quince
M’Barek Fatmi and Kerik D. Cox
CHAPTER 41. Detection of Pseudomonas syringae pv. actinidiae in Kiwifruit Plants
Angelo Mazzaglia and Giorgio M. Balestra
CHAPTER 42. Detection of Pseudomonas savastanoi pv. savastanoi in Asymptomatic Olive Plants
Stefania Tegli, Matteo Cerboneschi, and M’Barek Fatmi
CHAPTER 43. Detection of Agrobacterium spp. in Grapevines
Thomas J. Burr, Kameka L. Johnson, Supaporn Kaewnum, and Erno Szegedi
CHAPTER 44. Detection of Leifsonia xyli subsp. xyli in Sugarcane
Michael P. Grisham and Jeffrey W. Hoy
CHAPTER 45. Detection of Xanthomonas albilineans in Sugarcane
Freddy Fernando Garces, Jeffrey W. Hoy, and Michael P. Grisham
CHAPTER 46. Detection of Rhodococcus fascians in Ornamental Plants
Melodie L. Putnam
CHAPTER 47. Detection of Agrobacterium tumefaciens in Ornamental Plants
Melodie L. Putnam
CHAPTER 48. Detection of Burkholderia gladioli in Orchids
Lisa Keith and Piyarat Thammakijjawat