Flow Cytometry: A Practical Approach

About this book

Flow Cytometry 3/e is intended as a handbook for every laboratory that has a bench-top flow cytometer or a fluorescence activated cell sorter. It is an introduction and guide to those new to the field and a first point of reference for experienced practitioners who want to investigate a new technique. The chapter on immunophenotyping - the most important clinical application of flow cytometry - has been strengthened by the addition of a chapter on quality control in the clinical laboratory. The utility of the book in a clinical laboratory has been further enhanced by the addition of a chapter covering ten other clinical applications. Flow cytometry has found increasing application in the field of apoptosis research. A new chapter has been added to cover this important topic. Every flow cytometry laboratory can't afford not to have a copy on the shelf as a first point of reference. The book is not fully comprehensive but it does aim to cover over 90 per cent of the applications of flow cytometry in mammalian biology.

Contents

Introduction to the principles of flow cytometry; fluorescence and fluorescence technology; preparing of suspensions of single cells; flow sorting; immunofluorescence of surface markers; analysis of DNA - general methods; further clinical applications; quality control in the clinical laboratory; measurement of cytoplasmic and nuclear antigens; analysis of DNA-measurement of cell kinetics by the bromodeoxyuridine/anti-bromodeoxyuridine method; analysis of cell proliferation using the bromodeoxyuridine/Hoechst-propidium iodide method; chromosome analysis and sorting by flow cytometry; intracellular ionised calcium, magnesium, membrane potential and pH; flow cytometry in the study of apoptosis; further applications to cell and molecular biology.

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