High Throughput Phenotyping in Plants Methods and Protocols

About this book

Genetic approaches to understanding plant growth and development have always benefitted from screens that are simple, quantitative and rapid. Visual screens and morphometric analysis have yielded a plethora of interesting mutants and traits that have provided insight into complex regulatory pathways, and yet many genes within any given plant genome remain undefined. The premise underlying High Throughput Phenotyping in Plants: Methods and Protocols is that the higher the resolution of the phenotype analysis the more likely that new genes and complex interactions will be revealed.

The methods described in High Throughput Phenotyping in Plants: Methods and Protocols can be generally classified as quantitative profiling of cellular components, ranging from ions to small molecule metabolites and nuclear DNA, or image capture that ranges in resolution from chlorophyll fluorescence from leaves and time-lapse images of seedling shoots and roots to individual plants within a population at a field site.

Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, High Throughput Phenotyping in Plants: Methods and Protocols serves as an invaluable guide to plant researchers and all scientists who wish to better understand plant growth and development.

Contents

1. Image-Based Analysis of Light-Grown Seedling Hypocotyls in Arabidopsis Benjamin J. Cole and Joanne Chory
2. High Throughput Phenotyping of Plant Shoots Bettina Berger, Bas de Regt, and Mark Tester
3. High Throughput Phenotyping of Root Growth Dynamics Nima Yazdanbakhsh and Joachim Fisahn
4. LEAF GUI: Analyzing the Geometry of Veins and Areoles Using Image Segmentation Algorithms Charles A. Price
5. Remote Chlorophyll Fluorescence Measurements with the Laser Induced Fluorescence Transient (LIFT) Approach Roland Pieruschka, Denis Klimov, Joseph A. Berry, C. Barry Osmond, Uwe Rascher, and Zbigniew S. Kolber
6. Leaf Hue Measurements: A High Throughput Screening of Chlorophyll Content Laszlo Sass, Petra Majer, and Eva Hideg
7. High Resolution, Time-Lapse Imaging for Ecosystem-Scale Phenotyping in the Field Tim Brown, Christopher Zimmermann, Whitney Panneton, Nina Noah, and Justin Borevitz
8. High Throughput Phenotyping of Plant Populations Using a Personal Digital Assistant Raju Naik Vankudavath, Reddaiah Bodanapu, Yellamaraju Sreelakshmi, and Rameshwar Sharma
9. High Throughput Fractionation of Natural Products for Drug Discovery Ying Tu and Bing Yan
10. Conducting Molecular Biomarker Discovery Studies in Plants Christian Schudoma, Matthias Steinfath, Heike Sprenger, Joost T. van Dongen, Dirk Hincha, Ellen Zuther, Peter Geigenberger, Joachim Kopka, Karin Kohl, and Dirk Walther
11. Highly Sensitive High Throughput Profiling of Six Phytohormones Using MS-Probe Modification and Liquid Chromatography-Tandem Mass Spectrometry Mikiko Kojima and Hitoshi Sakakibara
12. Qualitative and Quantitative Screening of Amino Acids in Plant Tissues Will I. Menzel, Wen-Ping Chen, Adrian D. Hegeman, and Jerry D. Cohen
13. Arabidopsis thaliana Membrane Lipid Molecular Species and Their Mass Spectral Analysis Thilani Samarakoon, Sunitha Shiva, Kaleb Lowe, Pamela Tamura, Mary R. Roth, and Ruth Welti
14. ICP-MS as a Tool For High Throughput Analysis of Plants Javier Seravalli
15. The Plant Volatilome: Methods of Analysis Carlo Bicchi and Massimo Maffei
16. High Throughput Monitoring of Plant Nuclear DNA Contents via Flow Cytometry David W. Galbraith and Georgina M. Lambert
17. Transient RNAi Assay in 96-Well Plate Format Facilitates High Throughput Gene Function Studies in planta Shu-Zon Wu and Magdalena Bezanilla
18. A High Throughput Biological Conversion Assay for Determining Lignocellulosic Quality Scott J. Lee, Thomas A. Warnick, Susan B. Leschine, and Samuel P. Hazen
19. Carbohydrate Microarrays in Plant Science Jonatan U. Fangel, Henriette L. Pedersen, Silvia Vidal Melgosa, Louise I. Ahl, Armando Asuncion Salmean, Jack Egelund, Maja Gro Rydahl, Mads H. Clausen, and William G. T. Willats

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