Progenitor Cells Methods and Protocols

About this book

Progenitor cells have become important in regenerative medicine therapies, due to their potential to differentiate into many cell types. This capability, and understanding how to regulate the cells, will provide the basis for future cell therapies aimed at correcting tissue and organ dysfunction as a result of disease or injury. In, Progenitor Cells: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to investigate progenitor cells. These include methods and techniques of the manipulation of physical forces that shape progenitor cell behavior, studying progenitor cells in vivo, using non-mammalian and mammalian model systems, and investigating human progenitor cells, including their isolation, characterization and application in cell-based therapies.

Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Progenitor Cells : Methods and Protocols seeks to aid scientists in the further study progenitor cells and how they are studied across multiple systems.

Contents

1. Isolation of Satellite Cells from Single Muscle Fibres from Young, Aged or Dystrophic Muscles Valentina Di Foggia and Lesley Robson
2. Terminal Differentiation of Human Epidermal Stem Cells on Micro-patterned Substrates John T. Connelly
3. Isolation, Culture and Potentiality Assessment of Lung Alveolar Stem Cells Feride Oeztuerk-Winder and Juan-Jose Ventura
4. Three-dimensional In Vitro Culture Techniques for Mesenchymal Stem Cells Fatima A. Saleh, Jessica E. Frith, Jennifer A. Lee, Paul G. Genever
5. Isolation of Adult Stem cells and their Differentiation to Schwann Cells Cristina Mantovani, Giorgio Terenghi and Susan G Shawcross
6. Functional Purification of Human and Mouse Mammary Stem Cells Daniela Tosoni, Pier Paolo Di Fiore, Salvatore Pece
7. Isolation and Expansion of Endothelial Progenitor Cells Derived from Mouse Embryonic Stem Cells S.Bahram Bahrami, Mandana Veiseh, Nancy J.Boudreau

Section II. Non-mammalian Model Systems
8. Transcriptome Analysis of Drosophila Neural Stem Cells Katrina S. Gold and Andrea H. Brand
9. Live Imaging for Studying Asymmetric Cell Division in the C. elegans Embryo Alexia Rabilotta, Rana Amini, and Jean-Claude Labbe
10. Tol2-mediated Gene Transfer and in ovo Electroporation of the Otic Placode: A Powerful and Versatile Approach for Investigating Embryonic Development and Regeneration of the Chicken Inner Ear Stephen Freeman, Elena Chrysostomou, Koichi Kawakami, Yoshiko Takahashi, and Nicolas Daudet
11. Labelling Primitive Myeloid Progenitor Cells in Xenopus Ricardo Costa, Yaoyao Chen,Roberto Paredes, and Enrique Amaya
12. Identification of Oocyte Progenitor Cells in the Zebrafish Ovary Bruce W. Draper
13. FACS Analysis of the Planarian Stem Cell Compartment as a Tool to Understand Regenerative Mechanisms Belen Tejada Romero, Deborah J Evans and A. Aziz Aboobaker
14. Clonal and Lineage Analysis of Melanocyte Stem Cells (MSCs) and their Progeny in the Zebrafish Robert C. Tryon and Stephen L. Johnson
15. Reconstitution of the Central Nervous System During Salamander Tail Regeneration from the Implanted Neurospheres Levan Mchedlishvili, Vladimir Mazurov, Elly M. Tanaka
16. Following the Fate of Neural Progenitors by Homotopic/homochronic Grafts in Xenopus Embryos Raphael Thuret, Nancy Papalopulu

Section III. Mammalian Model Systems
17. Analysing the Angiogenic Potential of Gr-1+CD11b+ immature Myeloid Cells from Murine Wounds Elahe Mahdipour and Kimberly A. Mace
18. In vivo Imaging of Hematopoietic Stem Cells in the Bone Marrow Niche Oliver Barrett, Roberta Sottocornola and Cristina Lo Celso
19. Characterising the Phenotype of Murine Epidermal Progenitor Cells: Complementary Wholemount Visualisation and Flow Cytometry Strategies Korinna D. Henseleit, Ann P. Wheeler, Gary Warnes, and Kristin M. Braun
20. Murine Aggregation Chimeras and Wholemount Imaging in Airway Stem Cell Biology Ian R. Rosewell and Adam Giangreco
21. Live Imaging of Primitive Endoderm Precursors in the Mouse Blastocyst Joanna B. Grabarek and Berenika Plusa

Section IV. Human Systems
22. Utilising DNA Mutations to Trace Epithelial Cell Lineages in Human Tissues Sebastian Zeki, Trevor A Graham & Stuart A C McDonald
23. NF-Ya Protein Delivery As a Tool for Hematopoietic Progenitor Cell Expansion Alevtina D Domashenko, Susan Weiner, Stephen G Emerson
24. Exploring the Link Between Human Embryonic Stem Cell Organization and Fate using Tension-calibrated Extracellular Matrix Functionalized Polyacrylamide Gels Johnathon N. Lakins, Andrew R. Chin, and Valerie M. Weaver
25. Isolation of Circulating Angiogenic Cells Erin E. Vaughan and Timothy O'Brien
26. Methods for Characterisation / Manipulation of Human Corneal Stem Cells and their Applications in Regenerative Medicine Francesca Corradini, Beatrice Venturi , Graziella Pellegrini and Michele De Luca
27. ALDH as a Marker for Enriching Tumorigenic Human Colonic Stem Cells Anitha Shenoy, Elizabeth Butterworth, Emina H. Huang
28. Protocols for Investigating microRNA Functions in Human Neural Progenitor Cells Sandra Almeida, Celine Delaloy, Lei Liu, Fen-Biao Gao Francesca Corradini, Beatrice Venturi , Graziella Pellegrini and Michele De Luca

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