Extremophiles

About this book

This latest volume in the Methods in Microbiology series provides an overview of the methods used for the isolation, cultivation and handling of a wide variety of extremophiles, both at the stage of their isolation from natural samples and in pure culture. It contains protocols specific for work with extremophiles, as well as adaptations of "standard" microbiology protocols modified to enable the handling of extremophiles. Emphasis will be on detailed time-tested protocols that are specific for work with extremophiles and adaptations of "standard" microbiology protocols that have to be modified to be suitable for handling extremophiles.

Contents

THERMOPHILES
- In-situ activity studies in thermal environments
- The isolation of thermophiles from deep-sea hydrothermal environments
- Growth of hyperthermophilic microorganisms for physiological and nutritional studies
- Toward the large scale cultivation of hyperthermophiles at high temperature and high-pressure
- The chemotaxonomic analysis of lipids from extremophilic bacteria
- Membranes of thermophiles and other extremophiles
- Characterization and quantification of compatible solutes in (hyper)thermophilic microorganisms
- Functional genomics of the thermo-acidophilic archaeon Sulfolobus solfataricus
- Heat shock proteins in hyperthermophiles
- Deep-sea Thermococcales and their genetic elements: plasmids and viruses
- Genetic systems for Thermus
- Gene transfer systems for obligately anaerobic thermophilic bacteria
- Hyperthermophilic virus-host systems: detection and isolation
- Preservation of thermophilic microorganisms

PSYCHROPHILES
- Handling psychrophilic microorganisms
- Proteins from psychrophiles

ALKALIPHILES
- Cultivation of aerobic alkaliphiles
- Isolation, cultivation, and characterization of alkalithermophiles

ACIDOPHILES
- The isolation and study of acidophilic microorganisms

HALOPHILES
- Characterization of natural communities of halophilic microorganisms
- Cultivation of haloarchaea
- Halophile extraction from ancient crystals
- The assessment of the viability of halophilic microorganisms in natural communities
- Characterization of lipids of halophilic Archaea
- Characterization of organic compatible solutes of halotolerant and halophilic organisms
- Genetic systems for halophilic archaea
- The isolation of viruses of halophilic microorganisms
- Detection, quantification and purification of halocins: peptide antibiotics from haloarchaeal extremophiles
- The storage of halophilic bacteria

BAROPHILES
- Handling piezophilic microorganisms

RADIATION-RESISTANT MICROORGANISMS
- Measuring survival in microbial populations following exposure to ionizing radiation

STRICT ANAEROBES
- Strictly anaerobic microorganisms

APPLICATIONS OF EXTREMOPHILES
- Industrial screening of extemophiles for valuable biomolecules

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