Genome Mapping: A Practical Approach

About this book

Genome analysis has undergone a period of explosive growth in recent years. This book describes up-to-date methods for genome mapping, arranged in order of increasing resolution, from linkage analysis to restriction mapping. Topics covered include linkage mapping of human, animal, and plant genomes; mapping of quantitative trait loci; radiation hybrid mapping; HAPPY mapping; somatic cell hybrids; flow-sorted and microdissected chromosomes; fluorescence in situ hybridization; contig assembly; chromosome walking; and long-range restriction mapping. Two appendices describe sources of freely available biological and computational resources relevant to genome analysis.

Contents

1. Human linkage mapping; 2. Linkage mapping of plant and animal genomes; 3. Linkage mapping of quantitative trait loci in plants and animals; 4. Radiation hybrid mapping; 5. HAPPY mapping; 6. Construction and use of somatic cell hybrids; 7. The use of flow-sorted chromosomes in genome mapping; 8. The use of microdissected chromosomes in genome mapping; 9. Fluorescence in situ hybridization; 10. Contig assembly by fingerprinting; 11. Chromosome wallking; 12. Long-range restriction mapping of genomic DNA; Appendix 1 Core list of suppliers; Appendix 2 Non-commercial source for genome mapping; Appendix 3 Bioinformatics for genome mapping

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