MHC, Volume 2: A Practical Approach

About this book

The major histocompatibility complex is the subject of much research in the immunology area. There is interest in the structure of MHC proteins and in their function as antigen-presenting molecules. Many immunology labs are now faced with the prospect of tackling biochemical and genetic techniques in order to study these molecules, and these two "Practical Approach" volumes provide an introduction. MHC 2 is complemented by MHC 1.

Contents

Generation of HLA-specific human monoclonal antibodies; sequencing of proteins isolated by one or two dimensional gel electrophoresis; assembly of MHC molecules - requirements of glycosylation, oxidized glutathione, and peptides; expression, purification, and characterization of recombinant soluble MHC class I molecules; the use of pulsed field gel electrophoresis to map the human MHC; derivation and analysis of MHC transgenic mice; imaging of individual cell surface MHC antigens using fluorescent particles; sequence analysis of major histocompatibility molecules; approaches to studying MHC expression in mammals other than primates; techniques to identify the rules governing class II MHC-peptide formation.

Customer Reviews