Signalling by Inositides: A Practical Approach

About this book

The metabolism and functions of inositol phosphates impinge on various branches of biochemistry, physiology, and molecular biology, and methodological information is in consequence scattered far and wide. This book unites a selection of the most fundamental and commonly used techniques from leading international signal transduction laboratories, and brings together many valuable protocols for purifying and assaying inositides and related compounds. A novel feature is a catalogue of non-commercial sources of synthetic inositide analogues.

Contents

1. Assaying inositol phospholipid turnover in plant cells; 2. Measurement of inositol phosphate turnover in intact cells and cell-free systems; 3. Mass assay of inositol and its use to assay inositol polyphosphates; 4. Phospholipase C-gamma 1; 5. Baculovirus-promoted expression, purification, and functional assay of G-protein regulated PLC-beta isoenzymes; 6. Purification and assay of phospholipase C-delta; 7. The purification and assay of inositol polyphosphate-binding proteins; 8. Mass assay of inositol 1,4,5-trisphosphate and phosphatidylinositol 4,5-bisphosphate; 9. Desalting inositol polyphosphates by dialysis; 10. The Ins(1,4,5)P3 receptor; 11. Purification and assay of inositol hexakisphosphate kinase and diphosphoinositol pentakisphosphate kinase; 12. Phosphoinositide 3-kinase

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