Transgenesis Techniques Principles and Protocols

About this book

One of the major challenges currently facing the scientific community is to understand the function of the multitude of protein-coding genes that were revealed when the human genome was fully sequenced.

In Transgenesis Techniques: Principles and Protocols, Third Edition, experts in the field fully update and expand upon the previous edition in order to detail the transgenic techniques currently used to modify the genome. The volume explores classic procedures to genetically modify mice and other model organisms, as well as cutting-edge practices involving microinjection, site-specific recombination systems, cryopreservation, and many other topics. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to their subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.

Comprehensive and state-of-the-art, Transgenesis Techniques: Principles and Protocols, Third Edition is a comprehensive practical guide to the generation of transgenic animals and an invaluable source of information in any lab currently involved in transgenic techniques.

Contents

Part I: Transgenesis in Various Model Systems

1. Transgenesis in Drosophila melanogaster
Leonie Ringrose

2. Transgenesis in Caenorhabditis elegans
Matthias Rieckher, Nikos Kourtis, Angela Pasparaki, and Nektarios Tavernarakis

3. Transgenesis in Zebrafish with the Tol2 Transposon System
Maximiliano L. Suster, Hiroshi Kikuta, Akihiro Urasaki, Kazuhide Asakawa, and Koichi Kawakami

4. Generation of Transgenic Frogs
Jana Loeber, Fong Cheng Pan, and Tomas Pieler

5. Pronuclear DNA Injection for the Production of Transgenic Rats
Jean Cozzi, Ignacio Anegon, Val, Anne-CathessChristel Merrouche, and Yacine Cherifi

Part II: Transgenesis in the Mouse

6. Cell-Type Specific Transgenesis in the Mouse
James Gulick and Jeffrey Robbins

7. Transgene Design and Delivery into the Mouse Genome: Keys to Success
Lydia Teboul

8. Overexpression Transgenesis in Mouse: Pronuclear Injection
Wendy J.K. Gardiner and Lydia Teboul

9. Gene Targeting Vectors
J. Simon C. Arthur and Victoria A. McGuire

10. Gene Trap: Knockout on the Fast Lane
Melanie Ullrich and Kai Schuh

11. Culture of Murine Embryonic Stem Cells
Ivana Barbaric and T. Neil Dear

12. Targeting Embryonic Stem Cells
Roland H. Friedel

13. Generation of Chimeras by Microinjection
Anne Pl#ck and Christian Klasen

14. Generation of Chimeras by Morula Aggregation
Anne Pl#ck and Christian Klasen

15. Surgical Techniques for the Generation of Mutant Mice
Anne Pl#ck and Christian Klasen

16. Site-specific Recombinases for Manipulation of the Mouse Genome
Marie-Christine Birling, Fran#oise Gofflot, and Xavier Warot

17. Cre Transgenic Mouse Lines
Xin Wang

18. Large-Scale Mouse Mutagenesis
Elizabeth J. Cartwright

19. Dedicated Mouse Production and Husbandry
Lucie Vizor and Sara Wells

20. Biological Methods for Archiving and Maintaining Mutant Laboratory Mice: Part I - Conserving Mutant Strains
Martin D. Fray

21. Biological Methods for Archiving and Maintaining Mutant Laboratory Mice: Part II - Recovery and Distribution of Conserved Mutant Strains
Martin D. Fray

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