Antibodies: A Laboratory Manual

About this book

The now classic lab manual Antibodies, by Harlow and Lane, has been revised, extended, and updated by Edward Greenfield of the Dana-Farber Cancer Center, with contributions from other leaders in the field. This second edition of Antibodies is an essential resource for molecular biology, immunology, and cell culture labs on all matters relating to antibodies. The chapters on hybridomas and monoclonal antibodies have been recast with extensive new information and there are additional chapters on characterizing antibodies, antibody engineering, and flow cytometry. As in the original book, the emphasis in this second edition is on providing clear and authoritative protocols with sufficient background information and troubleshooting advice for the novice as well as the experienced investigator.

Contents

Preface

1 Antibody Production by the Immune System
    Stefanie Sarantopoulos
2 The Antibody Molecule
    Stefanie Sarantopoulos
3 Antibody-Antigen Interactions
    Stefanie Sarantopoulos
4 Antibody Responses
    Stefanie Sarantopoulos
5 Selecting the Antigen
    Edward A. Greenfield, James DeCaprio, and Mohan Brahmandam
6 Immunizing Animals
    Edward A. Greenfield
7 Generating Monoclonal Antibodies
    Edward A. Greenfield
8 Growing Hybridomas
    Edward A. Greenfield
9 Characterizing Antibodies
    Frances Weis-Garcia and Robert H. Carnahan
10 Antibody Purification and Storage
    Jordan B. Fishman and Eric A. Berg
11 Engineering Antibodies
    James Dasch and Amy Dasch
12 Labeling Antibodies
    Eric A. Berg and Jordan B. Fishman
13 Immunoblotting
    Larisa Litovchick
14 Immunoprecipiation
    James DeCaprio and Thomas O. Kohl
15 Immunoassays
    Thomas O. Kohl and Carl A. Ascoli
16 Cell Staining
    Scott J. Rodig
17 Antibody Screening using High Throughput Flow Cytometry
    Thomas D.l. Duensing and Susan R. Watson

Appendix I: Electrophoresis
Appendix II: Protein Techniques
Appendix III: General Information
Appendix IV: Bacterial Expression
Appendix V: Cautions
Index

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