Antibody Engineering: A Practical Approach

About this book

Recombinant DNA techniques have revolutionized the isolation and production of antibodies in recent years, and rapid changes have been made. This new Practical Approach book details protocols which allow the researcher to isolate a new antibody, analyse its properties, format the right antibody molecule or fragment, and produce it in usable quantities.

Contents

1. Construction and use of antibody gene repertoires; 2. Affinity maturation of antibodies using phage display; 3. Human antibody repertoires in transgenic mice: manupulation and transfer of YACs; 4. Measuring antibody affinities in solution; 5. Measuring antibody affinities using Bio-sensors; 6. Analysis of human antibody sequences; 7. Rodent to human antibodies by CDR grafting; 8. Rodent to human antibodies by phage display; 9. Choosing and manipulating effector functions; 10. Secretion and purification of antibody fragments expressed in bacteria; 11. Production of single chain Fv monomers and multimers; 12. Expression of immunoglobulin genes in mammalian cells; 13. In vitro linkage of antibody fragments into dimeric and multimeric forms

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