Glycovirology Protocols

About this book

Glycovirology Protocols reviews the increasing importance of glycosylation to the field of virology, as well as virus replication. The chapters provide an overview of glycosylation in relation to virus infection, and the generic techniques that are used to analyze and characterize glycoproteins.

Contents

Table of Contents; Chapter 1: Viruses and glycosylation: an overview -- Sugrue, R.J.; Chapter 2: M.E. Interaction between Respiratory Syncytial Virus and Glycosaminoglycans, including Heparan Sulfate. -- Hallak, L.K., Kwilas, S.A. and Peeples,; Chapter 3: Expression of the severe acute respiratory syndrome coronavirus 3a protein and the assembly of coronavirus-like particles in the Baculovirus expression system -- Khan, S., Ng, M.L. and Tan, Y.J.; Chapter 4: The C type lectins DC-SIGN and L-SIGN: receptors for viral glycoproteins -- Lozach, P.Y., Burleigh, L., Staropoli, I. and Amara. A.; Chapter 5: Functional analysis of the N-linked glycans within the fusion protein of respiratory syncytial virus -- Li, P. Rixon, H.W.McL., Brown, G. and Sugrue, R.J.; Chapter 6: Expression and purification of viral glycoproteins using recombinant Vaccinia Viruses for functional and structural Studies -- Li, Z.N. and Steinhauer, D.A.; Chapter 7: The use of 2-dimensional SDS PAGE to analyse the glycosylation heterogeneity of the respiratory syncytial virus (RSV) Fusion (F) Protein -- McDonald, T.P. and Sugrue, R.J.; Chapter 8: The use of monoclonal antibodies and lectins to identify changes in viral glycoproteins that are influenced by gltcosylation: The case of respiratory syncytial virus attachment (G) glycoprotein--Rawling, J. and Melero, J.A.; Chapter 9: Expression, glycosylation and modification of the spike (S) glycoprotein of severe acute respiratory syndrome coronavirus (SARS CoV) -- Shen, S., Tan, T.H.P. and Tan, Y.J.; Chapter 10: Analysis of glycoproteins of viruses in the family Bunyaviridae -- Shi, X. and Elliott, R.M.; Chapter 11: Secretion of respiratory syncytial virus F protein from insect cells -- Tan, B.H., Brown, G. and Sugrue, R.J.; Chapter 12: The expression and characterisation of recombinant dengue virus E protein expressed in Pichia pastoris. -- Tan, B.H., Fu, J.L. and Sugrue, R.J.; Chapter 13: Cloning, expression and functional analysis of patient-derived hepatitis C virus glycoproteins -- Tarr, A.W., Owsianka, A.M., Szwejk, A., Ball, K.K. and Patel, A.H.

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