This book describes a powerful and effective tool for the molecular biologist researcher. Readers will discover how DNA chromatography can be used in their own work. Intended as an introduction for the molecular biologist reader, this will also serve as a practical guide for the experienced user. The clear and concise writing style is also attractive to the chemist who wants to learn more about molecular biology.
Preface. Acknowledgement. About the Cover. Introduction. INSTRUMENTATION AND OPERATION. CHROMATOGRAPHIC PRINCIPLES FOR DNA SEPARATION. DHPLC. SIZE BASED SEPARATIONS. PURIFICATION OF NUCLEIC ACIDS. RNA CHROMATOGRAPHY. SPECIAL TECHNIQUES. LOOKING FORWARD. Appendix 1: Glossary of Terms. Appendix 2: System Cleaning and Passivation Treatment. Appendix 3: Frequently Asked DHPLC Questions. Index.