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Academic & Professional Books  Mycology

Microscopy & Fungi

Handbook / Manual
By: Marcel Lecomte(Author)
260 pages, colour photos, b/w illustrations
Microscopy & Fungi
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  • Microscopy & Fungi ISBN: 9782960345209 Edition: 6 Spiralbound Mar 2024 In stock
    £64.99
    #264910
Price: £64.99
About this book Contents Customer reviews Related titles

About this book

A comprehensive and detailed account of the equipment and techniques used in the microscopic study of fungi. The book includes useful practical advice, based on the wide experience of several well-known mycologists. Presented and written in an accessible style, the text is complemented by good illustrations and clear summary tables. Spiral bound to ensure the book will lay flat during use.

This is the first English language version of Microscopie & Champignons, now collated from the previous versions and parts thereof into a single corrected and enlarged sixth edition. The book should be invaluable to anyone wanting to develop their skills in fungal identification as well as incidentally providing something of an illustrated glossary of fungal terms.

Contents

Introduction(s) along all my seminars 8
      Our personal and educational targets 10
      A list of abbreviations 10
      About terminology 10

Part 1: the microscope 13
Which microscope to choose? 14
      Components of a microscope 14
      Refractive index (RI) 18
      Why use Immersion Oil? 20
      Some elements to guide your choice 21
      Our master purchase 23
      Procedure for carrying out a standard observation 23
Microscopy and photography 25
      Superimposing photographs 29
      Photography through a micrometric eyepiece 30
      Spores: drawings or photographs? 31
      Tools and storage flasks 34

Part 2: the basic equipment 35
First accessories 36
      Microscope glass blades (GB) and their storage 36
      Immersion oil 37
      Cleaning the eyepieces and objectives 37
      First observation 38
Observation media 39
      Fluid media 39
      Viscous media 41
      Types of microscopic preparations 43
      Extemporaneous microscopic preparations 43
      Semi-definitive preparations 43
      Assembly of uncoloured parts in definitive stained media 45
      Definitive preparations 45
      Definitive aqueous solvent media 46
      Definitive media with benzene or keto solvent 47
      A personal working protocol 49
Staining: limits and reality 50
      Different types of staining 50
      Nature and longevity of the dyes and reagents 52
      About solutions in chemistry 54
Dissociation 55
      Observation of fresh material 55
      Drigalski’s triangle 56
      Exsiccata’s observation 56
      Softeners 57
      A new softening liquid: G.S.M. 58
Protocol for performing a mycologic preparation 59
      Washing the preparations 59
      Improve the readability of a preparation 60
      Improve the dissociation of hard elements 61
      Cleaning the glass blades 62
Cuts for microscopy: a real problem 63
      Freehand cuts 63
      Freehand cuts with a support guide 64
      Ranvier’s microtome 64
      A new cutting device for Ranvier’s microtome 64
      Genat’s microtome 65
      Small automated laboratory microtomes 66
      A rapid inclusion’s technique in paraffin 67
      Leuckart’s bars 67
      Using epoxy resin for inclusion 68
Accidents of handling and problems with your preparations 77
      Becke’s line 82

Part 3: mushrooms under a microscope 83
      The secret life of a mushroom 84
Ascomycetes: asci and ascospores 85
      Ascomycetes: operculated or not? 87
      Reactions with iodine 88
      Little drops, vacuoles and nuclei 89
      Causing sporulation of an Ascomycete 92
      Causing the birth of some Ascomycetes 93
Basidiomycetes: basidia and basidiospores 95
      Some surprising basidia 97
      Siderophilic basidia 99
      A caulohymenium in the Boletales 101
      About basidiospores 104
      Reference models for the shape of the most common spores 105
      Spore’s shape and their ornamentation 106
      How measuring spores? 107
      Causing sporulation of a Basidiomycete 108
      A protocol to observe the gills of a Basidiomycete 109
      Spores and walls 110
      Protocol for spore’s observation 111
      Repetitive spores 112
      Chlamydospores 13
      Basidiomycetes : other conidia 114
      Spores and melzer 115
      Cystidia 116
      Observing hooked cystidia (on the gills of some Pluteus) 118
      Chrysocystidia 119
      Acrophysalides in Amanita 120
      Trabecular cystidia and pseudoparaphysis 121
      Cuticles 123
      Protocol for the observation of a cuticular coating 131
      Protocol for the observation of Basidiomycetes with partial or general veil 131
      Cuticular pigments 132
      Cuticular hairs 135
      Hymenial sebum 136
      Some special structures: acanthocytes and crystals 137
      Trama of gills in the Basidiomycetes 138
      Protocol for observing the trama of the Basidiomycetes 144
      Types of hyphae in the Basidiomycetes 145
      Protocol for observing the mushroom flesh 150
Aphyllophorales
      Types of hyphae in the Aphyllophorales 151
      Microscopy of the Polypores and Corticiaceae 163
      Boidin’s A.M.A. reaction in the Aphyllophorales 168
      Back to the hyphae (structure and growth) 169
      Different types of clamps (clamp connections) 172
      Carnivorous mushrooms (toxocysts and gliosphex) 173
      A special study of Hohenbuehelia atrocoerulea 177
Special staining and observation techniques 178
      Regressive staining 179
      Acid-resistant incrustations in the genus Russula 180
      Dermatocystidia in the genus Russula 183
Some dyes are too little used 185
      Cresyl blue 185
      Cyanophilia 187
      Study of the genus Lepiota (spores) 189
      Nigrosin 191
      Azo black (Chlorazol black) 193
      Giemsa’s mixture 94
      A novelty: the little blue pills 196
A double staining applied to the Basidiomycetes 198
      Congo red + phloxin B 198
      Azo black + phloxin B 200
      A new type of staining for Morchella sp. 201
Ammonia, a valuable chemical and a reagent 202
      Ammonia reaction in the genera Inocybe and Conocybe 202
      Crystals of calcium oxalate 203
      K.K.’s reaction in the genus Amanita 204
About Plants and Glomeromycetes: endomycorrhiza 206
      Plants and Glomeromycetes (spores) 210
      Orchidoid mycorrhiza 213
Parasitic fungi 215
      “Rusts” or Urediniomycetes 215
      “Coals” or Ustilaginomycetes 220
      Powdery mildews or Erisyphales 222
      Vine has many enemies 226
      Mildews 227
Moulds (generalities) 228
      Moulds under a microscope 230
      Moulds and growing media 232
      Moulds are not always our friends 235
      Yeasts 243
Mycological microscopy job aid 245
Checklist for a little mycophilic chemist 254
      General bibliography 259
      Addendum: some types of basidia 260

Customer Reviews

Handbook / Manual
By: Marcel Lecomte(Author)
260 pages, colour photos, b/w illustrations
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