Methods for General and Molecular Bacteriology

About this book

Long-awaited revision of the classic manual, covering all of the immense impacts molecular biology has had on bacteriology over the past decade.

Contents

Methodology for General and Molecular Microbiology; Morphology; Light microscopy; Determinative and cytological light microscopy; Electron microscopy; Cell fractionation; Antigen-antibody reactions; Growth: Physicochemical factors in growth; Nutrition and media; Enrichment and isolation; Solid, liquid/solid and semisolid culture; Liquid culture; Growth measurement; Culture preservation; Molecular Genetics: Gene mutation; Gene transfer in Gram-negative bacteria; Gene transfer in Gram-positive bacteria; Plasmids; Transposon mutagenesis; Gene cloning and expression; Polymerase chain reaction; Nucleic acid analysis; Metabolism: Physical analysis; Chemical analysis; Enzymatic activity; Permeability and transport; Systematics: Phenotypic characterization; DNA sequence similarities; Ribosomal RNA hybridization and gene sequencing; Nucleic acid probes; General Methods: Laboratory safety; Photography; Records and reports

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