Protein Microarrays Methods and Protocols

About this book

Progress in functional proteomics has been limited for a long time, partially caused by limitations in assay sensitivity and sample capacity; however, protein microarrays have the ability to overcome these limitations so that a highly parallel analysis of hundreds of proteins in thousands of samples is attainable. In "Protein Microarrays: Methods and Protocols", expert researchers in the field present an up-to-date collection of robust strategies in the field of protein microarrays and summarize recent advantages in the field of printing technologies, the development of suitable surface materials, as well as detection and quantification technologies. Written in the highly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, "Protein Microarrays: Methods and Protocols" aims to stimulate the application and further advancement of this powerful technology in labs worldwide.

Contents

Part I: Reverse Phase Protein Arrays

1. Reverse Phase Protein Microarrays for Clinical Applications
2. Impact of Blocking and Detection Chemistries on Antibody Performance for Reverse Phase Protein Arrays
3. Phosphoprotein Stability in Clinical Tissue and Its Relevance for Reverse Phase Protein Microarray Technology
4. Utilization of RNAi to Validate Antibodies for Reverse Phase Protein Arrays
5. Antibody-Mediated Signal Amplification for Reverse Phase Protein Array-Based Protein Quantification
6. Reverse-Phase Protein Lysate Microarray (RPA) for the Experimental Validation of Quantitative Protein Network Models
7. Characterization of Kinase Inhibitors Using Reverse Phase Protein Arrays
8. Use of Formalin-Fixed and Paraffin-Embedded (FFPE) Tissues for Diagnosis and Therapy in Routine Clinical Settings
9. Producing Reverse Phase Protein Microarrays from Formalin Fixed Tissues
10. Use of Reverse Phase Protein Microarrays to Study Protein Expression in Leukemia: Technical and Methodological Lessons Learned

Part II: Antibody Microarrays

11. Antibody Microarrays as Tools for Biomarker Discovery
12. Assessment of Antibody Specificity Using Suspension Bead Arrays
13. Quantitative Analysis of Phosphoproteins Using Microspot Immunoassays
14. Robust Protein Profiling with Complex Antibody Microarrays in a Dual-Color Mode
15. High-Throughput Studies of Protein Glycoforms Using Antibody-Lectin Sandwich Arrays
16. Microspot Immunoassay-Based Analysis of Plasma Protein Profiles for Biomarker Discovery Strategies
17. Recombinant Antibodies for the Generation of Antibody Arrays

Part III: Protein Microarrays

18. Producing Protein Microarrays from DNA Microarrays
19. Cell Arrays and High-Content Screening
20. Probing Calmodulin Protein-Protein Interactions Using High Content Protein Arrays
21. Protein Function Microarrays for Customized Systems-Oriented Proteome Analysis
22. Optimized Autoantibody Profiling on Protein Arrays

Part IV: Sample Immobilization Strategies

23. Inkjet Printing for the Production of Protein Microarrays
24. Impact of Substrates for Probe Immobilization
25. Contact Printing of Protein Microarrays

Customer Reviews