RNA Methodologies A Laboratory Guide for Isolation and Characterization

Contents

Preface. RNA and the Cellular Biochemistry Revisited. Transcription and the Organization of Eukaryotic Gene Sequences. Messenger RNA. Resilient Ribonucleases. RNA Isolation Strategies. Isolation of Polyadenylated RNA. Quality Control for RNA Preparations. Dot Blot Analysis. Electrophoresis of RNA. Photodocumentation and Image Analysis. The Northern Analysis. Nucleic Acid Probe Technology. Practical Nucleic Acid Hybridization. Principles of Detection. RT PCR. Messenger RNA Differential Display. Quantitative PCR Techniques. Quantification of Specific mRNAs by Nuclease Protection. Analysis of Nuclear RNA. An RNA Paradigm. Epilogue. A Few Pearls of Wisdom. Appendices: Phenol Preparation. Disposal of Ethidium Bromide and SYBR Green Solutions. DNase I Removal of DNA from an RNA Sample. RNase Incubation to Remove RNA from a DNA sample. Deionization of Formamide, Formaldehyde, and Glyoxal. Silanizing Centrifuge Tubes and Glassware. Centrifugation as a Mainstream Tool for the Molecular Biologist. Trypsinization Protocol for Anchorage-Dependent Cells. Isolation of High-Molecular-Weight DNA by the Salting-Out Procedure. RNA Isolation from Plant Tissue. Electrophoresis: Principles, Parameters, and Safety. Polyacrylamide Gels. Internal Controls. Trade Citations. Selected Suppliers of Equipment, Reagents, and Services. Glossary. Index.

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